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CHARACTERIZATION OF A FLUORESCENT PROTEIN REPORTER SYSTEM IBD

SANDRA J DIAS / AIR FORCE INSTITUTE OF TE
BIBLIOSCHOLAR
09 / 2012
9781249450603
Inglés

Sinopsis

Chemical and biological threats are ever present and attacks have occurred throughout the world in both war and peace-time. Multiple government agencies, academia, and private industry are developing detection capabilities to address such threats. The research presented in this paper supports development of a modular synthetic biologybased system that detects and reports the presence of a threat agent. Synthetic biology builds upon past research in genetic engineering and seeks to combine broad applications within biotechnology in novel ways. This basic research project will help to demonstrate a proof-of-concept design which will guide future studies on the development of a modular sensor platform. In this study, in vitro and in vivo techniques were used to evaluate a reporter system composed of the Tobacco Etch Virus (TEV) protease and an engineered fluorescent fusion protein for their combined effectiveness as a reporter system. The TEV protease demonstrated the ability to cleave the fusion protein construct to produce a measureable output signal. The coupling of this reporter system with a riboswitch recognition element showed that this system can be applied to detecting chemicals. Further studies to modify this recognition element suggest modularity for future biosensors to detect chemicals of military interest.This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact, and remains as true to the original work as possible. Therefore, you will see the original copyright references, library stamps (as most of these works have been housed in our most important libraries around the world), and other notations in the work.This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work.As a reproduction of a historical artifact, this work may contain missing or blurred pages, poor pictures, errant marks, etc. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.

PVP
17,78€